Journal: bioRxiv
Article Title: Characterization of cardiac pacemaker activity in the primate lemur Microcebus murinus
doi: 10.1101/2021.10.25.465774
Figure Lengend Snippet: A: APs recorded of SAN pacemaker myocytes from M. murinus under Tyrode’s superfusion. B: Spontaneous Ca 2+ transients recorded in SAN pacemaker myocytes under the control condition and after perfusion of Epi, ACh, ivabradine and ryanodine. C: Effect of Epi and ACh superfusion on the rate of APs (n=2 in Epi and n=2 in Ach) and Ca 2+ transients (n=2 in Epi and n=3 in Ach). D: Changes in the rate of Ca 2+ transients after perfusion of the IVA (n=4) and Ry (n=3). *p<0.05 by two-way Anova and paired T-test.
Article Snippet: Cells were then incubated for 1h at 37°C with primary antibodies against HCN4, (1:100, Alomone), sarcomeric alpha-actinin, clone EA-53 (1:100, Sigma-Aldrich), connexin 45 (1:100, H-85, Santa Cruz Biotechnology), Ca v 1.3 Ca 2+ channel polyclonal antibody (N38.8, neuromab UC Davis), cardiac troponin I (1:100, Millipore), caveolin 3 (1:100, BD Transduction Laboratories) and myogenine, (1:50, sc576 santa cruz).
Techniques: Control